Molecular mechanism of double Holliday junction dissolution
نویسندگان
چکیده
منابع مشابه
Top3α Is Required during the Convergent Migration Step of Double Holliday Junction Dissolution
Although Blm and Top3α are known to form a minimal dissolvasome that can uniquely undo a double Holliday junction structure, the details of the mechanism remain unknown. It was originally suggested that Blm acts first to create a hemicatenane structure from branch migration of the junctions, followed by Top3α performing strand passage to decatenate the interlocking single strands. Recent eviden...
متن کاملHolliday junction resolvases.
Four-way DNA intermediates, called Holliday junctions (HJs), can form during meiotic and mitotic recombination, and their removal is crucial for chromosome segregation. A group of ubiquitous and highly specialized structure-selective endonucleases catalyze the cleavage of HJs into two disconnected DNA duplexes in a reaction called HJ resolution. These enzymes, called HJ resolvases, have been id...
متن کاملThe HRDC domain of BLM is required for the dissolution of double Holliday junctions.
Bloom's syndrome is a hereditary cancer-predisposition disorder resulting from mutations in the BLM gene. In humans, BLM encodes one of five members of the RecQ helicase family. One function of BLM is to act in concert with topoisomerase IIIalpha (TOPO IIIalpha) to resolve recombination intermediates containing double Holliday junctions by a process called double Holliday junction dissolution, ...
متن کاملRecombination: Holliday Junction Resolution and Crossover Formation
The heterodimeric nuclease Mus81-Eme1 has been proposed to be a Holliday junction resolvase and has now been found to be responsible for nearly all meiotic crossovers in fission yeast. The intriguing substrate preference of this enzyme for nicked Holliday junctions opens the possibility that crossover formation may not always involve double Holliday junctions.
متن کاملCommentary All change at Holliday junction
It is probably fair to say that DNA structure holds center stage in many processes of genetic recombination. Understanding the conformation of branched DNA intermediates and their recognition and manipulation by proteins is the key to deducing the underlying mechanisms. In the original Holliday (1) model for homologous genetic recombination, the central intermediate is a four-way junction where...
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ژورنال
عنوان ژورنال: Cell & Bioscience
سال: 2014
ISSN: 2045-3701
DOI: 10.1186/2045-3701-4-36